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KMID : 0350519930460031125
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Journal of Catholic Medical College
1993 Volume.46 No. 3 p.1125 ~ p.1135
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Influence of Nonionic Contrast Media on Phagocytosis of the Granulocyte
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Abstract
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Granulocytes play an important role in a host defense mechanism. Iodinated contrast media, however, have been known to interfere the functions of leukocytes.
The aim of this study was to evaluate influence of osmolality and iodine concentration of non ionic contrast media and 0.4M NaCl on phagocytosis of human granulocytes. Granulocytes were purified from whole blood of 5 healthy adults. Six diluted
solutions of nonionic contrast media, i.e., iopromide, iopamidol, inhexol, ioversol, and 0.4 M NaCl were made respectively : undiluted, 1 : 2, 1 : 4, 1 : 8, 1 : 16 and 1 : 32 . After incubation of the mixtures of contrast media, granulocytes and
latex
particles, cytocentrifugation using Cytospin was done. Authors investigated the number of ingested latex particles and vacuoles within the granulocytes of the above mentioned solutions by a light microscope.
@ES The results were as follows :
@EN 1. Phagocytic activities of granulocytes on latex particles in NaCl and contrast media solutions were reduced significantly as osmolality increased.
2. All contrast media inhibited phagocytosis more than relatively hyperosmolal NaCl solutions in the same dilutions. .
3. No significant difference of phagocytic activity was observed between contrast media.
4. The number of vacuoles in granulocytes were not related with that of latex paticles and its distribution was irregular in everv dilutions.
The results of this study suggest that phagocytic activity of granulocytes was inhibited mainly by osmolality and partly by chemical constituents of nonionic contrast media. It is recommended to use low osmolal nonionic contrast media during
radiographic study using iodinated contrast media especially in patients with immunocompromised state or infectious disease.
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